Heparinase Enzymes
Heparinase I, Heparinase II and Heparinase III - isolated from natural strains of Flavobacterium heparinum.
For structural and disaccharide analysis of heparin and/or heparan sulphate.
Suitable for USP Chemical Tests / ⟨207⟩ 1,6-Anhydro Derivative for Enoxaparin Sodium.
Please email [email protected] for any questions, for a quote or to place and order
The multistep preparation method gives baseline chromatographic separation between Heparinase I, Heparinase II and Heparinase III.
The enzymes are stabilised with 0.1-0.2% BSA (Sigma A4378), 0.22uM-sterile filtered and delivered worldwide as frozen solutions.
International Units: The package size for the Heparinase enzymes is defined in International Units (IU) – one international unit is “the amount of enzyme that will form 1.0 μmole of unsaturated uronic acid per minute from a heparin or heparan sulphate substrate”.
Heparinase I (Hep I) EC 4.2.2.7
Degrades Heparin and the sulphated (S)-domains of HS by specific scission of the linkage between N-sulfated glucosamine (GlcN.SO3, +/-6-OSO3) and 2-O-sulfated iduronic acid (IdoA,2SO3); the elimination reaction yields di-and oligosaccharides with unsaturated, end-chain 2-O-sulphated uronic acids that absorb at 232nM.
Linkage Cleaved by Heparinase I
-4 GlcN.SO3 (+/-6SO3) 1 – 4 IdoA,2SO3 1-
Heparinase II (Hep II) EC number not assigned
Degrades Heparin and Heparan Sulphate
Catalyses the eliminative scission of the glycosidic linkages between N-sulfated or N-acetylated glucosamine and glucuronic or iduronic acid. This is a broad activity enzyme that tolerates O-sulfation of the uronic acid and glucosamine residues. It will also cleave amino sugar – uronic acid linkages where the amine is unsubstituted ie GlcNH3+ – GlcA/IdoA; these linkages are resistant to Hep I and Hep III.
Linkage cleaved by Hep II
-4 GlcN.R (+/-6SO3) 1 – 4 GlcA/IdoA (+/-2SO3) 1-
R is Acetyl (Ac) or SO3
Heparinase III (Hep III/Heparitinase) EC 4.2.2.8
Heparinase III (Hep III) degrades Heparan Sulphate (HS) in regions of low and intermediate sulfation and has very limited activity against heparin. The primary substrates for Hep III are N-acetylated or N-sulfated glucosamines linked to glucuronic acid (i.e. GlcNAc/GlcNSO3 1 – 4 GlcA). Hep III has weak activity on disaccharides containing Iduronic Acid (IdoA) and its action is blocked if the iduronate residue is sulfated at C-2 (IdoA, 2SO3). The highly sulphated regions in HS (the S-domains) are resistant to Hep III.
Linkage in HS cleaved by Hep III
-4 GlcN.R(+/-6SO3) 1-4 GlcA 1-
R is Acetyl (Ac) or SO3
Product | Catalog No | Qty | £ |
---|
Heparinase I - 0.1 IU | HEP-ENZ I | 0.1 IU | 154.00 | Enquire Now | |
Heparinase I - 0.5 IU | HEP-ENZ I 0.5IU | 0.5 IU | 440.00 | Enquire Now | |
Heparinase I - 2.0 IU | HEP-ENZ I 2.0IU | 2.0 IU | 850.00 | Enquire Now | |
Heparinase I - 5.0 IU | HEP-ENZ I 5.0IU | 5.0 IU | 1900.00 | Enquire Now |
Heparinase II - 0.1 IU | HEP-ENZ II | 0.1 IU | 484.00 | Enquire Now | |
Heparinase II - 0.5 IU | HEP-ENZ II 0.5IU | 0.5 IU | 2200.00 | Enquire Now | |
Heparinase II - 1.0 IU | HEP-ENZ II 1.0IU | 1.0 IU | 3993.00 | Enquire Now |
Heparinase III - 0.1 IU | HEP-ENZ III | 0.1 IU | 319.00 | Enquire Now | |
Heparinase III - 0.5 IU | HEP-ENZ III 0.5IU | 0.5 IU | 880.00 | Enquire Now | |
Heparinase III 1.0 IU | HEP-ENZ III 1.0IU | 1.0 IU | 1540.00 | Enquire Now | |
Heparinase III - 2.0 IU | HEP-ENZ III 2.0IU | 2 IU | 2750.00 | Enquire Now |
Are you interested in a product? or you need some information? Don't hesitate to get in touch.