Heparin Oligosaccharides and Desulphated Heparin Oligosaccharides

Heparin Oligosaccharides

High-purity Heparin Oligosaccharides from dp4 to dp24

Prepared by the controlled, partial depolymerisation of heparin by Heparinase I and separation of the derived oligosaccharides using high-resolution gel filtration.  

General formula: *UA,2S – (GlcNS,6S – IdoA,2S)n=1-9 – GlcNS,6S

*the uronic acid at the non-reducing end of the chain contains a C4-C5 double bond due to the endolytic action of Heparinase I

Although the main disaccharide in heparin is the trisulphated unit GlcNS,6S – IdoA,2S (approx. 65%), heparin also contains di-, mono- and non-sulphated units; thus each size class of oligo, though substantially homogeneous in molecular size, contains structures that vary in content and pattern of sulphation.

In the product list below dp = degree of polymerisation of monosaccharides. So a dp4 (HO04) is a tetrasaccharide, a dp6 (HO06) is a hexasaccharide, a dp8 (HO08 )is an octasaccharide………etc.

Desulphated Heparin Oligosaccharides 

Prepared by partial depolymerisation of desulphated heparins by Heparinase enzymes and separation of the derived oligosaccharides using high resolution gel filtration.

3 variations of selectively desulphated heparin oligos are available, sizes dp4 – dp12

2-O-deS oligo: sulphate group removed from the C2 of iduronate

6-O-deS oligo: sulphate group removed from the C6 of glucosamine

N-deS/Ac oligo: amino group in N-deS heparin modified by acetylation

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